Very little. For each microbiome sample, transfer only enough material to reach the indicated fill line on the vial. Do not underfill or overfill the tube, as an incorrect amount may prevent the lab from processing your sample and could require recollection with a new kit.
For the blood sample, leave the Tasso device attached while the indicator dots fill from the top. If all four dots fill and you can see blood at the bottom of the sample pod, stop the timer and gently peel the device from your arm. Otherwise, remove the device after five minutes. If you see the sample pod mostly full after five minutes, you may also keep the device on for an extra minute to ensure enough blood sample is collected. Ideally most, if not all, dots are filled before removal. Do not open the sample pod or attempt to add more blood manually.
Following the collection instructions carefully helps ensure the laboratory receives enough material to process your samples accurately.